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    CURRENT ARTICLE by Karl Simpson


HIV, TB and Malaria kill well over 6 million human beings each year. That is a nation the size of Switzerland. Unfortunately those 6 million deaths have little economic impact on deciders in the rich world for whom the threat of bioterrorism (perhaps 6 dead!), easily unlocks multi-billion dollar defense allocations. Other infections probably kill or contribute to the death of 100s of millions of human beings each year.

 

Infectious disease‚ the return of vaccines?

The 20th century was a period of great revolutions in medicine, none more impressive than the remarkable victories scored against infectious disease. The discovery of antibiotics in the first half of the century gave hope to those who died of bacterial infections related to childbirth, or wounds or simply ambient microbes. Concurrently novel vaccines were offering long-term protection from both viral and bacterial illnesses. In the latter half of the century smallpox was eradicated and as we enter the 21st century polio may soon join smallpox as just a terrible memory for older people.

Despite the successes against bacteria and viruses, the battle against fungal and parasitic disease never did more than maintain an uneasy state of war.

Today the hard-won victories against bacteria and viruses are being threatened by the increasing number of microbes able to resist the current range of antibiotics. As many as 30% of people who enter hospitals in the developing world acquire antibiotic-resistant infections, while being treated for something else.

Vaccines seem to be maintaining their usefulness, but despite our vast recent progress in understanding life, very few new vaccines have been authorized for use. The problem here is that the regulatory agencies have no clear protocols in place with which to determine the safety and efficacy of vaccines. Unlike old fashioned chemical agents, complex biological molecules are effective only in their target species ‚ man. Tests in animals have almost no relevance to the human condition or likely therapeutic outcome. Some progress has been made in generating transgenic animals as surrogates for human beings. However, these present terribly limited models of human immunity, and do not take into account the wider range of human-specific metabolic activities.

Viruses, bacteria and other infections

HIV, TB and Malaria are a trio of nasty agents that illustrate the opportunities and difficulties faced by the panoply of anti-infective strategies.

• HIV is a virus causing AIDS

• Tuberculosis is a disease caused by bacterium

• And Malaria is a disease caused by a protozoan parasite transmitted by mosquito bites

HIV is a group of viruses belonging to the family of retroviruses. These retroviruses carry their genetic information in the form of double stranded RNA. The RNA of retroviruses is associated with an enzyme known as Reverse Transcriptase. The RNA and reverse transcriptase are surrounded by a cone-shaped protein shell or capsid. The capsid itself is surrounded by another shell, the virus envelope, which is a complex of lipids and proteins.

Once inside a target cell, a retrovirus makes a DNA copy of the RNA using the reverse transcriptase enzyme (so called because it works in a manner which is the reverse of the normal DNA goes to RNA copying - or transcription). Inside the cell most of the virus's genome exists as DNA. This will then intercalate itself into the hosts chromosomal DNA.

In the case of HIV, the virus recognizes, and specifically attacks, cells containing a certain marker on the surface. This marker, known as an antigen, is called for convenience CD4. HIV infects cells which carry the CD4 antigen. In man among the cells carrying the CD4 antigen are key cells of the immune system; lymphocytes and other white blood cells. HIV infection thus causes damage to the immune system and stops the infected person from eliminating that infection. The body tries hard however, but in the mid or long term (2-15 years), HIV infection left untreated is usually fatal.

Tuberculosis is caused by the bacterium, Mycobacterium tuberculosis (M. tuberculosis). It is quite closely related to the agent of leprosy, Mycobacterium leprae (M.leprae). Most mycobacteria are harmless soil organisms, but several have become pathogens of man and animals. The mycobacteria share several antigens and an attenuated form of the agent causing tuberculosis of cattle, M. bovis, is used to vaccinate against M. tuberculosis. This is the well-known BCG vaccine. A TB infection can also be treated with appropriate antibiotics, but increasingly resistance to these drugs is becoming encountered.

In addition to antibiotic resistance HIV is giving a new stimulus to tuberculosis, for it is in patients with a damaged immune system that M. tuberculosis becomes an opportunistic infection. Thus TB is a further complication of the current AIDS epidemic.

Malaria is a disease caused by a protozoan parasite of the genus Plasmodium. Four principal parasites are recognized: P. vivax, P. malariae, P. ovale and P. falciparum. All four Plasmodium species have a two-host life cycle, alternating between man and mosquitoes of the genus Anopheles. There has as yet been no successful vaccine against Malaria. Quinine derived chemicals have shown to be very effective at preventing Malaria infection, but increasingly Plasmodium species are showing resistance to these and other drugs. Successful treatment of Malaria can take several forms. The cycle of transmission can be broken by destroying Anopheles mosquitoes, the insect host of Plasmodium. New drugs can be developed which interfere with the complicated reproductive cycle of Plasmodium. Finally a vaccine can be developed, allowing the human host to recognize and eliminate new Plasmodium infections. There has been progress in vaccine development, but the consensus today is that an effective vaccine is still far from reality.

Lessons from these agents.

All three of these agents can illustrate the unfortunate division of society into rich world and poor world. And this too is unfortunate, for the lessons to be learned from poor world disease could be applied to the very many illnesses affecting man in the rich world‚ which might be caused by infectious agents.

HIV first came to prominence in the homosexual community of the rich world in the early 1980s. Poor hygiene in the rich-world drug abusing community further encouraged the spread of HIV viruses. Now over twenty years later it has been recognized that HIV comes in at least two viral forms and is not just a scourge of homosexuals and drug addicts. The threat to the economy of the rich world generated solutions ‚ of a sort ‚ for HIV infection. Unfortunately these solutions, based on agents which inhibit viral replication (nucleotide analogues and viral protease inhibitors) are in themselves dangerous, and furthermore only control the infection. Recipients of famous triple therapy will relapse if the therapeutic regime is interrupted. In the developing world, and particularly in Africa, promiscuous heterosexual sex is the primary cause of the spread of HIV. Over 40 million persons have been infected according to the World Health Organization (twice that number according to some estimates). It is not clear to what extent malnutrition and other infections enhance the transmission of HIV.

Tuberculosis was more or less eliminated from the rich world by about 1970. It continued to exist only in the poorest communities and as a disease caught on exotic holidays in foreign places. It was treatable. No longer! The drugs no longer work well and HIV has helped TB recapture much or all the ground lost in the first half of the 20th century. In the rich world we ignore the fact that, in much of the poor world, TB continued unabated throughout the last century. That fact was brought home to me when I lived in Hong Kong in the 1960s.

During the years of colonial occupation, the major colonizing powers all came face to face with the threat of Malaria. By draining swamps and controlling mosquitoes, significant progress was made. Quinine derivatives became available and palatable (to this we owe the gin and tonic) preventive agents. Simple physical barriers such as mosquito nets and well built houses could reduce the threat.

All the above and the many other infectious and parasitic diseases compromised economic growth. Such was the importance of our colonies that we developed solutions, if not cures, for the problems presented. With the independence of the colonies these concerns ceased to worry the former masters. And the diseases came back, until the global community under the auspices of the United Nations and specifically the World Health Organization recognized that pools of disease were but a short flight away from our rich economies. Action was needed.

What is infectious disease?

In the above lines we identified three specific agents of disease, representing viral, bacterial and parasitic infections.

However there are many more disease states for which no specific agent has been recognized. In some cases this is because the disease state is congenital, due to a genetic or environmental stress that resulted in abnormal development. Other diseases can be due to environmental agents such as pollutants or toxins. Accidents are in some respects a simple physical interaction with the environment.

Clearly there are diseases which have nothing‚ or little‚ to do with infectious agents, but it may be surprising just how many illnesses are due wholly or in part to infectious agents. Any weakness or prolonged illness will compromise the immune system, leaving the body vulnerable to infection.

Some kinds of breast cancer may be caused by viruses. Other types of cancer may be in part inherited, but an environmental trigger‚ such an infectious agent‚ can directly provoke the cancer (an example of this is Burkitt's lymphoma, triggered by Epstein-Barr virus).

It seems possible that many inflammatory illnesses may be due to infection with small bacteria or mycoplasmas. These are not easily diagnosed and are so omnipresent that differentiation of harmless and pathological species may be next to impossible.

Relationships with infectious agents

At school we all learned about parasites, symbionts and commensals. Parasites live in or on us and harm us. Symbionts live in or on us and pay for the ride by actively helping us. Commensals appear to hitch a ride doing neither good nor harm. Those are fairly simplistic definitions, but they are workable.

And they confuse the perception that all bacteria and viruses are bad. Some bacteria are essential. Without them humans could not absorb essential vitamins from the gut (which contains kilogrammes of bacteria!). Viruses; surely they are all bad? No! Some viruses may play a role in controlling bacteria which might otherwise harm us. Others may even play a role in monitoring and controlling cancer. But surely we all learned that viruses are pathological agents. True in the 1950s, but now we know much more. Ordinary cells may communicate in part through the exchange of virus like particles. When does a virus-like particle become a virus? Consider even the tapeworm (disgusting), but in some parts of the world a tapeworm more than pays its way by preventing the establishment of other parasites that could do real harm.

Why vaccines are important

Vaccines are a cost-effective solution to disease. One or a few treatments can secure life-long protection from disease. Some vaccines may actively combat established infections or disease states, by recognizing antigens on infected cells and killing them and their pathological content or alerting the immune system.

Modern vaccines may be expensive to develop, but even priced at ¤ 250 a treatment, this represents a huge cost saving on days of productive work lost, not to mention the cost of active medical interventions.

Although there seems little incentive to develop a vaccine which helps poor people far away in irrelevant countries, it is just those poor people that constitute a danger for you and me in the rich world.

• Firstly, they may harbour diseases which travel to infect us or which we may catch on vacation in exotic destinations (and return home to our families and friends).

• Secondly, poor people constitute a reservoir of have-nots who eye our wealth jealously. For those who have nothing violence is a sure way to attract attention. America learned this lesson in September last year.

• Thirdly, health is the first pre-requisite of a competitive workforce. The second is education, but that can only work if children or adults are well enough to follow a course of learning. Vaccines deliver health that allows them to study. Healthy workers make a healthy economy and if we are to grow richer we must generate new markets in the developing world. The developing world can only become a market if its people can pay. Vaccines deliver the health that allows them to pay the bills.

Population densities in many parts of the world are higher than ever before. These high densities favor the emergence of new infectious diseases, like HIV, or the re-emergence of old diseases we have nearly forgotten - like TB. Malaria too is re-emerging and this is due primarily to our neglect. Let us attempt to stop this potential wave of destruction.

Yes disease affects poor people far away, but it is terrifying how rapidly the spectre of endemic infection and ill health could come to haunt us again.

Biological weapons‚ a sick joke?

Yes we should be concerned about biological weapons. However, the only good thing to come out of the present scares is the realization that we can all be struck down by infection. A world prepared to fight infectious disease through heavy investment in vaccine research and development is a world ready for biological weapons. Remember last year biological weapons killed 6 people. HIV, TB and Malaria alone killed 6 million and the other infections collectively killed perhaps 100 million. Let us get a grip on reality! It is Mother Nature who controls the biological weapons, not the terrorists!